999 HUMAN CORD BLOOD: LYMPHOCYTE SUBPOPULATIONS AND LYMPHOKINE PRODUCTION

ALTERATION OF T-LYMPHOCYTE SUBPOPULATIONS IN SUBACUTE AND CHRONIC BRUCELLOSIS

Human brucellosis is a major health problem in developing countries. A number of patients with human brucellosis do not recover from the acute stage of the disease and pass into the chronic form. Since the pathophysiology of this change is not well understood, we studied the T-cell subsets during the acute, subacute and chronic forms of human brucellosis. In this study we found alterations...

Beta chemokines costimulate lymphocyte cytolysis, proliferation, and lymphokine production.

We report here the ability of the beta chemokines MIP-1 alpha, MIP-1 beta, RANTES, and MCP-1 to enhance some lymphocyte effector functions. Initial studies focused on the effects of chemokines on human and mouse cytotoxic T lymphocyte (CTL)- and natural killer (NK) cell-specific cytolytic responses. The results demonstrate that beta chemokines are capable of augmenting mouse and human CTL and h...

Lymphokine(s) from isolated T lymphocyte subpopulations support multilineage hematopoietic colony and megakaryocytic colony formation.

Conditioned medium derived from peripheral mononuclear low-density cells stimulated with phytohemagglutinin (PHA) supports the growth of noncommitted hematopoietic progenitors from marrow and peripheral blood cells. These immature progenitors (CFU-GEMM) can be identified in culture as multilineage hematopoietic colonies containing erythroblasts, eosinophilic, basophilic and neutrophilic granulo...

RNA content in human lymphocyte subpopulations.

Human peripheral blood lymphocytes are stained with the metachromatic dye acridine orange and the fluorescence of individual cells is measured by flow cytometry. The relative content of stainable RNA per cell is estimated by comparison with RNase-treated cells. Non-T and T lymphocytes have different mean quantities of RNA per cell, and these classes exhibit different distributions of RNA conten...

Lymphocyte subpopulations.